Please use this identifier to cite or link to this item: https://apo.ansto.gov.au/dspace/handle/10238/4116
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dc.contributor.authorPatwardhan, SV-
dc.contributor.authorHolt, SA-
dc.contributor.authorKelly, SM-
dc.contributor.authorKreiner, M-
dc.contributor.authorPerry, CC-
dc.contributor.authorvan der Walle, CF-
dc.date.accessioned2012-04-11T23:32:59Z-
dc.date.available2012-04-11T23:32:59Z-
dc.date.issued2010-09-29-
dc.identifier.citationPatwardhan, S. V., Holt, S. A., Kelly, S. M., Kreiner, M., Perry, C. C., van der Walle, C. F., (2011). Silica Condensation by a Silicatein α Homologue Involves Surface-Induced Transition to a Stable Structural Intermediate Forming a Saturated Monolayer. Biomacromolecules, 11(11), 3126-3135en_AU
dc.identifier.govdoc3856-
dc.identifier.issn1525-7797-
dc.identifier.urihttp://dx.doi.org/10.1021/bm100932een_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/4116-
dc.description.abstractSilicatein alpha exists within the protein filament of silica spicules of the marine sponge Tethya aurantium in a predominantly beta-sheet structure. However, it is produced in a soluble form with mixed alpha-helix/beta-sheet structure akin to its cathepsin L homologue. To understand this conformational transition in the context of enzyme catalyzed silica condensation, we used a functional, recombinant silicatein alpha termed 4SER. In solution, 4SER becomes conformationally unstable at pH 7 and readily unfolds to a soluble beta-sheet intermediate, losing the majority of its helical structure. This beta-sheet intermediate is present following adsorption of 4SER to a silica surface from solution. 4SER is particularly surface active, forming a near saturated monolayer on SiO(2) from low bulk concentrations, without transition to multilayers at high bulk concentrations. The adsorbed intermediate remains stable during silica condensation and drying. We propose that the beta-sheet structure for silicatein a. in marine sponge spicules represents a stable structural intermediate, formed upon adsorption to the silica surface. Copyright © 2010 American Chemical Societyen_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectSILICAen_AU
dc.subjectPROTEINSen_AU
dc.subjectSolar prominencesen_AU
dc.subjectENZYMESen_AU
dc.subjectFILAMENTSen_AU
dc.subjectDehydrocyclizationen_AU
dc.titleSilica Condensation by a Silicate in alpha Homologue Involves Surface-Induced Transition to a Stable Structural Intermediate Forming a Saturated Monolayeren_AU
dc.typeJournal Articleen_AU
dc.date.statistics2012-02-12-
Appears in Collections:Journal Articles

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