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|Title:||Quantitative elemental localisation in leaves and stems of nickel hyperaccumulating shrub Hybanthus floribundus subsp floribundus using micro-PIXE spectroscopy|
|Citation:||Kachenko, A. G., Singh, B., Bhatia, N. P., & Siegele, R. (2008). Quantitative elemental localisation in leaves and stems of nickel hyperaccumulating shrub Hybanthus floribundus subsp floribundus using micro-PIXE spectroscopy. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, 266(4), 667-676. doi:10.1016/j.nimb.2007.11.053|
|Abstract:||Hybanthus floribundus (Lindl.) F.Muell. subsp. floribundus is a native Australian nickel (Ni) hyperaccumulating shrub and a promising species for rehabilitation and phytoremediation of Ni tailings. Spatial localisation and quantification of Ni in leaf and stem tissues of H. floribundus subsp.,floribundus was studied using micro-proton-induced X-ray emission (micro-PIXE) spectroscopy. Young plants, grown in a potting mix under controlled glasshouse conditions were exposed to Ni concentrations of 0 and 26 mM kg(-1) for 20 weeks. Leaf and stem samples were hand-sectioned and freeze-dried prior to micro-PIXE analysis. Elemental distribution maps of leaves revealed Ni concentration of 7800 mg kg(-1) dry weight (DW) in whole leaf sections, which was identical to the bulk tissue analysis. Elemental maps showed that Ni was preferentially localised in the adaxial epidermis (10,000 mg kg(-1) DW) and reached a maximum of up to 10,000 mg kg(-1) DW in the leaf margin. Freeze-dried stem sections from the same plants contained lower Ni than leaf tissues (1800 mg kg(-1) versus 7800 mg kg(-1) DW, respectively), however did not resolve a clear pattern of compartmentalisation across different anatomical regions. Our results suggest localisation in epidermal cells is an important physiological mechanism involved in Ni accumulation and tolerance in leaves of H. floribundus subsp. floribundus. © 2007, Elsevier Ltd.|
|Gov't Doc #:||1456|
|Appears in Collections:||Journal Articles|
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