Browsing by Author "Skoda, MWA"
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- ItemAsymmetric phospholipid: lipopolysaccharide bilayers; a Gram-negative bacterial outer membrane mimic(The Royal Society, 2013-10-16) Clifton, LA; Skoda, MWA; Daulton, E; Hughes, AV; Le Brun, AP; Lakey, JH; Holt, SAThe Gram-negative bacterial outer membrane (OM) is a complex and highly asymmetric biological barrier but the small size of bacteria has hindered advances in in vivo examination of membrane dynamics. Thus, model OMs, amenable to physical study, are important sources of data. Here, we present data from asymmetric bilayers which emulate the OM and are formed by a simple two-step approach. The bilayers were deposited on an SiO2 surface by Langmuir–Blodgett deposition of phosphatidylcholine as the inner leaflet and, via Langmuir–Schaefer deposition, an outer leaflet of either Lipid A or Escherichia coli rough lipopolysaccharides (LPS). The membranes were examined using neutron reflectometry (NR) to examine the coverage and mixing of lipids between the bilayer leaflets. NR data showed that in all cases, the initial deposition asymmetry was mostly maintained for more than 16 h. This stability enabled the sizes of the headgroups and bilayer roughness of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and Lipid A, Rc-LPS and Ra-LPS to be clearly resolved. The results show that rough LPS can be manipulated like phospholipids and used to fabricate advanced asymmetric bacterial membrane models using well-known bilayer deposition techniques. Such models will enable OM dynamics and interactions to be studied under in vivo-like conditions. © 2013, The Royal Society.
- ItemExamining the creation and destruction of model bacterial surfaces(International Conference on Neutron Scattering, 2017-07-12) Clifton, LA; Skoda, MWA; Hughes, A; Holt, SA; Lakey, JBacteria are differentiated into two main groups, Gram-positive or Gram-negative, based on the Gram stain which detects the thick peptidoglycan cell wall of gram positive bacteria.Gram-negative bacteria are of particular biomedical and technological interest due to their role in disease, the increasing antibiotic resistance if some species and their utility in many biotechnological processes. The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS). LPS is a polyanionic molecule, with numerous phosphate groups present in the Lipid A and core oligosaccharide regions. We have attempted to create GNB-OM assays which are amenable to molecular level characterisation. These systems are asymmetric phospholipid : lipopolysaccharide membranes deposited at the solid/liquid interface and consist of either solid supported bilayers at the silicon/water interface or floating supported bilayers at the gold/water interface. The analysis of these membrane models by neutron reflectometry has provided new insights into the OM and interactions with it. Examples of this include providing conformation of the activity of anti-bacterial proteins, the role of the lipopolysaccharide polysaccharide chains in protecting the bacterium and the importance of divalent cations in stabilising the OM structure.
- ItemThe relationship between charge density and polyelectrolyte brush profile using simultaneous neutron reflectivity and in situ attenuated total internal reflection FTIR(American Chemical Society, 2013-05-21) Topham, PD; Glidle, A; Toolan, DTW; Weir, MP; Skoda, MWA; Barker, R; Howse, JRWe report on a novel experimental study of a pH-responsive polyelectrolyte brush at the silicon/D2O interface. A poly[2-(diethylamino)ethyl methacrylate] brush was grown on a large silicon crystal which acted as both a substrate for a neutron reflectivity solid/liquid experiment but also as an FTIR-ATR spectroscopy crystal. This arrangement has allowed for both neutron reflectivities and FTIR spectroscopic information to be measured in parallel. The chosen polybase brush shows strong IR bands which can be assigned to the N?D+ stretch, D2O, and a carbonyl group. From such FTIR data, we are able to closely monitor the degree of protonation along the polymer chain as well as revealing information concerning the D2O concentration at the interface. The neutron reflectivity data allows us to determine the physical brush profile normal to the solid/liquid interface along with the corresponding degree of hydration. This combined approach makes it possible to quantify the charge on a polymer brush alongside the morphology adopted by the polymer chains. © 2013, American Chemical Society.