Browsing by Author "Reinhardt, J"

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    High speed free-run ptychography at the Australian Synchrotron
    (Australian Nuclear Science and Technology Organisation, 2021-11-26) Kewish, CM; Jones, MWM; van Rissen, GA; Phillips, NW; Hinsley, GN; Schrank, CE; Afshar, J; Reinhardt, J; de Jonge, MA
    The Australian Synchrotron X-ray Fluorescence Microscopy (XFM) beamline has recently implemented fast scanning ptychography, a scanning X-ray diffraction microscopy method. Ptychography creates super-resolution images from transmitted microdiffraction patterns acquired as the sample is scanned through the beam. Highspeed detectors and high-performance computers are required to iteratively reconstruct these complex images. The experimental methods and reconstruction algorithms have significantly evolved over the last decade and a half into a mature and user-friendly complementary imaging method to XFM. Here we present the implementation of high speed ptychography at the XFM beamline, which includes a free run data collection mode where detector dead time is eliminated, and the scan time is optimized. We show that free-run data collection is viable for fast and high-quality ptychography by demonstrating extremely high data rate acquisition covering areas up to 352,000 μm2 at up to 140 μm2/s, with 18× spatial resolution enhancement compared to the beam size. With these improvements, ptychography at velocities up to 250 μm/s is approaching speeds compatible with fast-scanning X-ray fluorescence microscopy. The combination of these methods provides morphological context for elemental and chemical information, enabling unique scientific outcomes. © The Authors
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    High-speed free-run ptychography at the Australian Synchrotron
    (International Union of Crystallography, 2022-03) Jones, MWM; van Riessen, GA; Phillips, NW; Schrank, CE; Hinsley, GN; Afshar, N; Reinhardt, J; de Jonge, MD; Kewish, CM
    Over the last decade ptychography has progressed rapidly from a specialist ultramicroscopy technique into a mature method accessible to non-expert users. However, to improve scientific value ptychography data must reconstruct reliably, with high image quality and at no cost to other correlative methods. Presented here is the implementation of high-speed ptychography used at the Australian Synchrotron on the XFM beamline, which includes a free-run data collection mode where dead time is eliminated and the scan time is optimized. It is shown that free-run data collection is viable for fast and high-quality ptychography by demonstrating extremely high data rate acquisition covering areas up to 352 000 μm2 at up to 140 μm2 s-1, with 13x spatial resolution enhancement compared with the beam size. With these improvements, ptychography at velocities up to 250 μm s-1 is approaching speeds compatible with fast-scanning X-ray fluorescence microscopy. The combination of these methods provides morphological context for elemental and chemical information, enabling unique scientific outcomes. © The Authors - Open Access CC-By Licence
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    Simultaneous reconstruction and structural fitting of the complex atomic fine structure of copper and iron
    (Australian Institute of Physics, 2022-12-11) Di Pasquale, P; Tran, CQ; Chantler, CT; Barnea, Z; Kirk, T; Dao, MN; Balaur, E; van Riessen, GA; Hinsley, GN; Jallandhra, A; Ceddia, J; Rogers, J; Kewish, CM; Paterson, DJ; Reinhardt, J; Kirby, N; Mudie, ST
    A novel technique for determining complex atomic fine structure will be described. Exciting applications of the technique such as a phase analogue to x-ray absorption fine structure applications will also be discussed.
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    Synchrotron x-ray fluorescence microscopy-enabled elemental mapping illuminates the ‘battle for nutrients’ between plant and pathogen
    (Oxford University Press, 2021-03-29) Naim, F; Khambatta, K; Sanglard, LMVP; Sauzier, G; Reinhardt, J; Paterson, DJ; Zerihun, A; Hackett, MJ; Gibberd, MR
    Metal homeostasis is integral to normal plant growth and development. During plant–pathogen interactions, the host and pathogen compete for the same nutrients, potentially impacting nutritional homeostasis. Our knowledge of outcome of the interaction in terms of metal homeostasis is still limited. Here, we employed the X-ray fluorescence microscopy (XFM) beamline at the Australian Synchrotron to visualize and analyse the fate of nutrients in wheat leaves infected with Pyrenophora tritici-repentis, a necrotrophic fungal pathogen. We sought to (i) evaluate the utility of XFM for sub-micron mapping of essential mineral nutrients and (ii) examine the spatiotemporal impact of a pathogen on nutrient distribution in leaves. XFM maps of K, Ca, Fe, Cu, Mn, and Zn revealed substantial hyperaccumulation within, and depletion around, the infected region relative to uninfected control samples. Fungal mycelia were visualized as thread-like structures in the Cu and Zn maps. The hyperaccumulation of Mn in the lesion and localized depletion in asymptomatic tissue surrounding the lesion was unexpected. Similarly, Ca accumulated at the periphery of the symptomatic region and as microaccumulations aligning with fungal mycelia. Collectively, our results highlight that XFM imaging provides the capability for high-resolution mapping of elements to probe nutrient distribution in hydrated diseased leaves in situ. © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology.