Browsing by Author "Mume, E"

Now showing 1 - 6 of 6
  • No Thumbnail Available
    Item
    Investigating the binding properties of porous drug delivery systems using nuclear sensors (radiotracers) and positron annihilation lifetime spectroscopy – predicting conditions for optimum performance
    (Royal Society Chemistry, 2011-01-01) Mume, E; Lynch, DE; Uedono, A; Smith, SV
    Understanding how the size, charge and number of available pores in porous material influences the uptake and release properties is important for optimising their design and ultimately their application. Unfortunately there are no standard methods for screening porous materials in solution and therefore formulations must be developed for each encapsulated agent. This study investigates the potential of a library of radiotracers (nuclear sensors) for assessing the binding properties of hollow silica shell materials. Uptake and release of Cu2+ and Co2+ and their respective complexes with polyazacarboxylate macrocycles (dota and teta) and a series of hexa aza cages (diamsar, sarar and bis-(p-aminobenzyl)-diamsar) from the hollow silica shells was monitored using their radioisotopic analogues. Coordination chemistry of the metal (M) species, subtle alterations in the molecular architecture of ligands (Ligand) and their resultant complexes (M-Ligand) were found to significantly influence their uptake over pH 3 to 9 at room temperature. Positively charged species were selectively and rapidly (within 10 min) absorbed at pH 7 to 9. Negatively charged species were preferentially absorbed at low pH (3 to 5). Rates of release varied for each nuclear sensor, and time to establish equilibrium varied from minutes to days. The subtle changes in design of the nuclear sensors proved to be a valuable tool for determining the binding properties of porous materials. The data support the development of a library of nuclear sensors for screening porous materials for use in optimising the design of porous materials and the potential of nuclear sensors for high through-put screening of materials. © 2011, Royal Society of Chemistry
  • No Thumbnail Available
    Item
    Optimizing radiolabeling amine-functionalized silica nanoparticles using SarAr-NCS for applications in imaging and radiotherapy
    (American Chemical Society, 2013-05-07) Kong, L; Mume, E; Triani, G; Smith, SV
    Silica nanoparticles functionalized with amine groups and in the size range of approximately 60–94 nm were produced by combining sol–gel processing and emulsion technology. Hexa-aza cage ligand SarAr-NCS was conjugated to the silica nanoparticles and subsequently radiolabeled with a solution of 57Co2+-doped carrier Co2+. The number of Co2+ ions bound to the silica particles at pH 7 was used to determine the average number of available SarAr-NCS ligands conjugated to a silica particle. For organically modified silica particles of 94.0 and 59.5 nm diameter, the maximum number of metal binding sites was determined to be 11700 and 3270 sites per particle, respectively. For silica particles (63.5 nm peak diameter) produced using an water-in-oil emulsion, the calculated average was 4480 on the particle surface. The number of SarAr-NCS conjugated on the particles was easily controlled, potentially providing for a range of products for applications in the risk assessment of particles and theranostic imaging or radiotherapy when radiolabeled with a suitable radioisotope such as 64Cu or 67Cu. © 2013, American Chemical Society.
  • No Thumbnail Available
    Item
    Peptide modification of purified gellan gum
    (Royal Society of Chemistry, 2014-12-10) Ferris, CJ; Stevens, LR; Gilmore, KJ; Mume, E; Greguric, I; Kirchmajer, DM; Wallace, GG; in het Panhuis, M
    AbstractGellan gum (GG) is an anionic polysaccharide with potential as a biopolymer for additive manufacturing (3D-bioprinting) and tissue engineering. Previous studies have shown GG to be highly cytocompatible, but lacking specific attachment sites required for anchorage-dependent cells. In this work, we modify purified-GG polymer with a short peptide containing the arginine-glycine-aspartic acid (RGD) sequence that is known to enhance integrin-mediated cell attachment. Radiolabelling of the peptide was used in optimisation of the conjugation procedure to achieve an overall efficiency of 40%. The purification of divalent cations from commercial GG samples was found to be critical for successful conjugation. Rheological studies revealed that the peptide coupling did not prevent gelation behaviour. C2C12 cells showed improved attachment on the surface of and encapsulated within RGD-GG hydrogels, differentiating to multinucleated myofibers after 5–7 days. PC12 cells showed minimal interactions with both GG and RGD-GG, with formation of cell clusters and impedance of terminal differentiation and neurite extension. © 2015 Royal Society of Chemistry
  • No Thumbnail Available
    Item
    Plasmonic light yield enhancement of a liquid scintillator
    (AIP Publishing LLC, 2013-05-28) Bignell, LJ; Mume, E; Jackson, TW; Lee, GP
    We demonstrate modifications to the light yield properties of an organic liquid scintillator due to the localization of the tertiary fluorophore component to the surface of Ag-core silica-shell nanoparticles. We attribute this enhancement to the near-field interaction of Ag nanoparticle plasmons with these fluor molecules. The scintillation light yield enhancement is shown to be equal to the fluorescence enhancement within measurement uncertainties. With a suitable choice of plasmon energy and scintillation fluor, this effect may be used to engineer scintillators with enhanced light yields for radiation detection applications. © 2013, AIP Publishing LLC.
  • Thumbnail Image
    Item
    Role of positron annihilation lifetime studies and nuclear sensors for characterising porous materials
    (Institute of Physics, 2010-08-01) Mume, E; Uedono, A; Mizunaga, G; Lynch, DE; Smith, SV
    A series of nuclear sensors were designed to assess the chemistry within the nanopores of a porous material. The nuclear sensors of varying size, charge, and hydrophobicity were exposed to hollow silica shells (HSS) at varying pH. Uptake and release kinetics were studied over a 24 h period at room temperature. Preliminary study indicate positively charged nuclear sensors were selectively and rapidly (within 10 min) absorbed by the HSS at pH 7 to 9. PALS showed there were two types of pores (1.7 and 0.7 nm) present. The data suggest the nuclear sensors sit within the larger pore of the HSS. Both PALS and nuclear sensors are required to obtain an accurate insight into the nanoporosity of the hollow silica shells.
  • No Thumbnail Available
    Item
    Synthesis of hexa aza cages, SarAr-NCS and AmBaSar and a study of their metal complexation, conjugation to nanomaterials and proteins for application in radioimaging and therapy
    (Royal Society of Chemistry, 2013-07-01) Mume, E; Asad, A; Di Bartolo, NM; Kong, L; Smith, C; Sargeson, AM; Price, R
    A novel hexa aza cage, N1-(4-isothiocyanatobenzyl)-3,6,10,13,16,19-hexaazabicyclo[6.6.6]icosane-1,8-diamine (SarAr-NCS) was synthesized in good yield and characterized by 1H NMR and electrospray mass spectrometry. A new method for the synthesis of the related N1-(4-carboxybenzyl)-3,6,10,13,16,19-hexaazabicyclo[6.6.6]icosane-1,8-diamine (AmBaSar) using the p-carboxybenzaldehyde is reported. The complexation of Cu2+, Co2+ and Zn2+ by the two ligands over a range of pHs was found to be similar to the parent derivative SarAr. SarAr-NCS was conjugated to both silica particles (≈90 nm diam.) and the model B72.3 murine antibody. The SarAr-NCSN-silica particles were radiolabeled with Cu2+ doped 64Cu and the number of ligands conjugated was calculated to be an average of 7020 ligands per particle. Conjugation of SarAr-NCS to the B72.3 antibody was optimized over a range of conditions. The SarAr-NCSN-B72.3 conjugate was stored in buffer and as a lyophilized powder at 4 °C over 38 days. Its radiolabeling efficiency, stability and immunoreactivity were maintained. The development of a high yielding synthesis of SarAr-NCS should provide an entry point for a wide range of Cu and Zn radiometal PET imaging agents and potentially radiotherapeutic agents with 67Cu. © 2013, The Royal Society of Chemistry.