Browsing by Author "Harrisson, S"

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    In vivo tracking of dendritic cells in patients with multiple myeloma
    (Lippincott Williams & Wilkins, 2008-02-01) Prince, HM; Wall, DM; Ritchie, D; Honemann, D; Harrisson, S; Quach, H; Thompson, M; Hicks, RJ; Lau, EW; Davison, J; Loudovaris, M; Bartholeyns, J; Katsifis, A; Mileshkin, L; Moloney, J; Loveland, B
    Dendritic cell (DC) immunotherapy is being actively studied in multiple myeloma (MM). We aimed to use positron emission tomography or single positron emission tomography to determine the in vivo distribution of monocyte-derived nonmatured DC or matured DC (mDC) administered to patients with MM. Eligible patients had stable or slowly progressive MM and elevated serum MUC-1 or MUC-1 expression on marrow plasma cells. DCs were derived from granulocyte-macrophage colony-stimulating factor+ interleukin-13 stimulated autologous monocytes, pulsed with mannan-MUC1 fusion protein, and matured by FMKp and interferon-gamma. Before injection, DCs were labeled with either 18fluorine-fluorodeoxyglucose, 111indium-oxine or 64copper-pyruvaldehyde-bis-N-4-methylthiosemicarbazone. Labeled DCs were given either as a single intravenous dose or by concurrent subcutaneous (SC), intradermal (ID), and intranodal routes. 18Fluorine-fluorodeoxyglucose tracking was unsuccessful owing to high radiolabel efflux. 64Copper-pyruvaldehyde-bis-N-4-methylthiosemicarbazone-labeled mDC (n=2 patients) demonstrated tracking to regional nodes but quantitation was also limited owing to cellular efflux. 111Indium-oxine, however, gave reproducible tracking of both nmDc and mDC (n=6) to regional lymph node after either SC or ID administration, with mDC revealing superior migration to regional lymph node. SC and ID routes produced similar levels of DC migration. © 2008 by Lippincott Williams & Wilkins
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    Pore size and volume effects on the incorporation of polymer into macro- and mesoporous zirconium titanium oxide membranes
    (American Chemical Society, 2009-12) Drisko, GL; Cao, L; Kimling, MC; Harrisson, S; Luca, V; Caruso, RA
    Macro- and mesoporous hybrid materials have applications in the fields of drug delivery, catalysis, biosensing, and separations. The pore size requirements must be well-understood to maximize the performance (e.g., load capacity and accessibility) of such materials. Hybrid materials were prepared by coating five distinct macroporous commercial membranes with zirconium titanium oxide through sol−gel chemistry. Calcination of these templated materials produced oxide membranes which had a suite of macropore and mesopore architectures, pore volumes, and surface areas. These differences in physical properties were used to conduct a fundamental study on the relationship between the pore size and volume and the polymer incorporation. Metal oxide membranes were postsynthetically modified with poly(ethyleneimine) (PEI) ranging in molecular weight from 1300 to 1000000 Da (1.2−11 nm in hydrodynamic diameter). The incorporation of the polymer from a 9 wt % solution at pH 10 was highly dependent on the pore size and pore volume. As the surface area increased, loading capacity decreased, indicating that much of the increased internal surface, due to small pore diameters (≤8 nm), was inaccessible to the macromolecules. Exclusion of PEI from small mesopores was apparent even for the lowest molecular weight polymer. A high maximum loading of 1.25 mg m−2 of 600000−1000000 Da PEI was achieved in the metal oxide with the largest minimum mesopore diameter. Thus, mesopore diameter and pore volume must be considered when designing a mesoporous solid support. © 2009, American Chemical Society