Browsing by Author "García, ÁL"

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    Langmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayer
    (Springer Nature, 2021-12) Cranfield, CG; Le Brun, AP; García, ÁL; Cornell, BA; Holt, SA
    Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182–189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers. © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.