The 18 kDa translocator protein (peripheral benzodiazepine receptor) expression in the bone of normal, osteoprotegerin or low calcium diet treated mice

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dc.contributor.authorKam, WWYen_AU
dc.contributor.authorMeikle, SRen_AU
dc.contributor.authorDunstan, CRen_AU
dc.contributor.authorBanati, RBen_AU
dc.contributor.authorBlair, JMen_AU
dc.contributor.authorZheng, Yen_AU
dc.date.accessioned2020-03-23T23:55:09Zen_AU
dc.date.available2020-03-23T23:55:09Zen_AU
dc.date.issued2012-01-25en_AU
dc.date.statistics2020-03-20en_AU
dc.description.abstractThe presence of the translocator protein (TSPO), previously named as the mitochondrial or peripheral benzodiazepine receptor, in bone cells was studied in vitro and in situ using RT-qPCR, and receptor autoradiography using the selective TSPO ligand PK11195. In vitro, the TSPO is highly expressed in osteoblastic and osteoclastic cells. In situ, constitutive expression of TSPO is found in bone marrow and trabecular bone, e.g., spongiosa. Mice with a reduction of bone turnover induced by a 4-day treatment of osteoprotegerin reduces [3H]PK11195 binding in the spongiosa (320±128 Bq.mg−1, 499±106 Bq.mg−1 in saline-treated controls). In contrast, mice with an increase in bone turnover caused by a 4-day low calcium diet increases [3H]PK11195 binding in the spongiosa (615±90 Bq.mg−1). Further, our study includes technical feasibility data on [18F]fluoride microPET imaging of rodent bone with altered turnover. Despite [18F]fluoride having high uptake, the in vivo signal differences were small. Using a phantom model, we describe the spillover effect and partial volume loss that affect the quantitative microPET imaging of the small bone structures in experimental mouse models. In summary, we demonstrate the expression of TSPO in small rodent bone tissues, including osteoblasts and osteoclasts. A trend increase in TSPO expression was observed in the spongiosa from low to high bone turnover conditions. However, despite the potential utility of TSPO expression as an in vivo biomarker of bone turnover in experimental rodent models, our small animal PET imaging data using [18F]fluoride show that even under the condition of a good biological signal-to-noise ratio and high tracer uptake, the currently achievable instrument sensitivity and spatial resolution is unlikely to be sufficient to detect subtle differences in small structures, such as mouse bone. © 2012 Plos Oneen_AU
dc.identifier.articlenumbere30623en_AU
dc.identifier.citationKam, W.W. Y., Meikle, S. R., Zhou, H., Zheng, Y., Blair, J. M., & Banati, R. B. (2012). The 18 kDa translocator protein (peripheral benzodiazepine receptor) expression in the bone of normal, osteoprotegerin or low calcium diet treated mice. PloS one, 7(1), e30623. doi:10.1371/journal.pone.0030623en_AU
dc.identifier.govdoc8774en_AU
dc.identifier.issn1932-6203en_AU
dc.identifier.issue1en_AU
dc.identifier.journaltitlePloS oneen_AU
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0030623en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/9208en_AU
dc.identifier.volume7en_AU
dc.language.isoenen_AU
dc.publisherPLOS ONEen_AU
dc.subjectProteinsen_AU
dc.subjectReceptorsen_AU
dc.subjectMiceen_AU
dc.subjectCalciumen_AU
dc.subjectBone tissuesen_AU
dc.subjectLigandsen_AU
dc.subjectPositron computed tomographyen_AU
dc.titleThe 18 kDa translocator protein (peripheral benzodiazepine receptor) expression in the bone of normal, osteoprotegerin or low calcium diet treated miceen_AU
dc.typeJournal Articleen_AU
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