Evaluation of [I-123]-CLINDE as a potent SPECT radiotracer to assess the degree of astroglia activation in cuprizone-induced neuroinflammation

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dc.contributor.authorMattner, Fen_AU
dc.contributor.authorBandin, DLen_AU
dc.contributor.authorStaykova, Men_AU
dc.contributor.authorBerghofer, PJen_AU
dc.contributor.authorGrégoire, MCen_AU
dc.contributor.authorBallantyne, Pen_AU
dc.contributor.authorQuinlivan, Men_AU
dc.contributor.authorFordham, Sen_AU
dc.contributor.authorPham, TQen_AU
dc.contributor.authorWillenborg, DOen_AU
dc.contributor.authorKatsifis, Aen_AU
dc.date.accessioned2013-11-27T04:39:57Zen_AU
dc.date.available2013-11-27T04:39:57Zen_AU
dc.date.issued2011-08-01en_AU
dc.date.statistics2013-11-27en_AU
dc.description.abstractThe purpose of this study was to assess the feasibility and sensitivity of the high-affinity translocator protein (TSPO) ligand [123I]-CLINDE in imaging TSPO changes in vivo and characterise and compare astroglial and TSPO changes in the cuprizone model of demyelination and remyelination in C57BL/6 mice. Methods C57BL/6 mice were fed with cuprizone for 4 weeks to induce demyelination followed by 2–4 weeks of standard diet (remyelination). Groups of mice were followed by in vivo single photon emission computed tomography (SPECT)/CT imaging using [123I]-CLINDE and uptake correlated with biodistribution, autoradiography, immunohistochemistry, immunofluorescence and real-time polymerase chain reaction (RT-PCR). Results The uptake of [123I]-CLINDE in the brain as measured by SPECT imaging over the course of treatment reflects the extent of the physiological response, with significant increases observed during demyelination followed by a decrease in uptake during remyelination. This was confirmed by autoradiography and biodistribution studies. A positive correlation between TSPO expression and astrogliosis was found and both activated astrocytes and microglial cells expressed TSPO. [123I]-CLINDE uptake reflects astrogliosis in brain structures such as corpus callosum, caudate putamen, medium septum and olfactory tubercle as confirmed by both in vitro and in vivo results. Conclusion The dynamics in the cuprizone-induced astroglial and TSPO changes, observed by SPECT imaging, were confirmed by immunofluorescence, RT-PCR and autoradiography. The highly specific TSPO radioiodinated ligand CLINDE can be used as an in vivo marker for early detection and monitoring of a variety of neuropathological conditions using noninvasive brain imaging techniques. © 2011, Springer.en_AU
dc.identifier.citationMattner, F., Bandin, D. L., Staykova, M., Berghofer, P., ,Grégoire M. C., Ballantyne, P., Quinlivan, M., Fordham, S., Pham, T., Willenborg, D. O., & Katsifis, A. (2011). Evaluation of [I-123]-CLINDE as a potent SPECT radiotracer to assess the degree of astroglia activation in cuprizone-induced neuroinflammation. (2011). European Journal of Nuclear Medicine and Molecular Imaging, 38(8), 1516-28. doi:10.1007/s00259-011-1784-2en_AU
dc.identifier.govdoc5294en_AU
dc.identifier.issn1619-7070en_AU
dc.identifier.issue8en_AU
dc.identifier.journaltitleEuropean Journal of Nuclear Medicine and Molecular Imagingen_AU
dc.identifier.pagination1516-1528en_AU
dc.identifier.urihttp://dx.doi.org/10.1007/s00259-011-1784-2en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5038en_AU
dc.identifier.volume38en_AU
dc.language.isoenen_AU
dc.publisherSpringeren_AU
dc.subjectSingle photon emission computed tomographyen_AU
dc.subjectMiceen_AU
dc.subjectProteinsen_AU
dc.subjectCorrelationsen_AU
dc.subjectAutoradiographyen_AU
dc.subjectChain reactionsen_AU
dc.titleEvaluation of [I-123]-CLINDE as a potent SPECT radiotracer to assess the degree of astroglia activation in cuprizone-induced neuroinflammationen_AU
dc.typeJournal Articleen_AU
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