Determining glucose metabolism kineticsuUsing 18F-FDG micro-PET/CT

This paper describes the use of 18F-FDG and micro-PET/CT imaging to determine in vivo glucose metabolism kinetics in mice (and is transferable to rats). Impaired uptake and metabolism of glucose in multiple organ systems due to insulin resistance is a hallmark of type 2 diabetes. The ability of this technique to extract an image-derived input function from the vena cava using an iterative deconvolution method eliminates the requirement of the collection of arterial blood samples. Fitting of tissue and vena cava time activity curves to a two-tissue, three compartment model permits the estimation of kinetic micro-parameters related to the 18F-FDG uptake from the plasma to the intracellular space, the rate of transport from intracellular space to plasma and the rate of 18F-FDG phosphorylation. This methodology allows for multiple measures of glucose uptake and metabolism kinetics in the context of longitudinal studies and also provides insights into the efficacy of therapeutic interventions. © 2022 MyJoVE Corporation

Keywords

Glucose, Metabolism, Fluorine 18, Fluorine isotopes, Positron computed tomography, Diabetes mellitus, Rats, Phosphorylation, CAT scanning

Citation

Cochran, B. J., Ryder, W. J., Parmar, A., Klaeser, K., Reilhac, A., Angelis, G. I., Meikle, S. R., Barter, P. J., & Rye, K.-A. (2017). Determining glucose metabolism kineticsuUsing 18F-FDG micro-PET/CT, JoVE: Journal of Visualized Experiments (123), e55184. doi:10.3791/55184